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Previous research on boar ejaculate freezability biomarkers mainly focused on proteomics. To solve this issue, researchers are engaged in a lot of work to distinguish good (GFE) and poor freezability ejaculates (PFE). Therefore, it is meaningful to distinguish between high and low freezability individuals before cryopreservation procedures and to select high freezability individuals for cryopreservation to improve the efficiency of artificial insemination utilizing post-thawed sperm. On the other hand, the quality of frozen-thawed boar semen shows strong variability in freezability between individuals. On one hand, boar spermatozoa in general presents low freezability because of its high cold shock sensitivity. However, frozen–thawed boar semen accounts for less than 1% of the semen used for insemination. D-aspartic acid, NAG, and inosine in seminal plasma may be potential markers for assessing sperm cryopreservation resistance in boars.Īrtificial insemination has been widely used in pig production worldwide. In conclusion, there is significant difference in the metabolome of seminal plasma between GFE and PFE individuals.
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Further, twelve metabolites were subjected to metabolic target analysis, and three metabolites (D-aspartic acid, N-acetyl-L-glutamate (NAG), and inosine) showed differences. A total of 953 metabolites were identified in boar semen plasma by UHPLC-qTOF-MS analysis, and 50 metabolites showed significant change between the GFE group and PFE group. Therefore, the main aim of this study was to explore the differences in the metabolic level of seminal plasma between boars with differential freezability and to explore the candidate biomarkers of semen freezability.
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However, details and significance of the seminal plasma metabolome related to boar sperm freezability are unknown. The exposure of spermatozoa to small molecules such as metabolites can affect sperm function. The seminal plasma is composed of secretions from the testis, epididymis, and accessory sex glands. Ludwig Simmet's legacy will be preserved and perpetuated in the future - thanks to the efforts of around 400 dedicated employees of Minitube companies around the world who are committed to developing new and continuously improved technologies in animal reproduction.Some potential markers of boar sperm freezability have been found in spermatozoa, but little attention has been paid to seminal plasma. Aiming to make a valuable contribution to the production of healthy and sustainable food, alone more than 130 employees are currently working at the headquarters in Tiefenbach in the areas of research and development, production, quality management, the production of pharmaceutical media, sales and logistics, among others.Īlthough the planned celebration of the company's 50th anniversary had to be postponed by one year due to the restrictions imposed by the corona crisis, Dr.
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#MINITUBE BOAR SEMEN FREEZING PROFESSIONAL#
Steadily growing turnover and employee numbers, numerous patents, independent certifications and awards, as well as the worldwide acknowledged professional expertise attest to the crisis-proof orientation of the company, especially in times of a global economic recession. Today, Minitube with ten subsidiaries worldwide is a leading specialist supplier for the animal breeding industry and has remained a family-owned business, in which the third generation has now joined the management.